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Henderson Hasselbalch Calculator
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The Henderson Hasselbalch equation was developed independently by the American biological chemist L.J.Henderson and the Swedish physiologist K.A. Hasselbalch for relating the pH to the bicarbonate buffer system of the blood.

The Henderson Hasselbalch equation describes the relationship between dissociated and undissociated acid or base.

The equivalent forms of Henderson Hasselbalch equation are
pH = pKa + log$\frac{[A^-]}{[HA]}$
or
pH = pKa + log$\frac{[Base]}{[Acid]}$
or
pH = -log[Ka] + log$\frac{[A^-]}{[HA]}$

Here pKa is -log[Ka]. Where Ka is the acid dissociation constant.
 

Steps for Henderson Hasselbalch Calculator

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Step 1 :  

Read the problem and list out the values given.



Step 2 :  

Substitute the values in the corresponding formula to obtain the pH value.


pH = -log[Ka] + log$\frac{[A^-]}{[HA]}$



Problems on Henderson Hasselbalch Calculator

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  1. To mimic a blood buffer, a scientist prepared 1.000L buffer containing 0.0025mol carbonic acid and 0.025mol hydrogen carbonate ion. Calculate the pH of the buffer. The Ka of carbonic acid is 4.2 $\times$ 10-7.


    Step 1 :  

    Given data


    Ka = 4.2 $\times$ 10-7


    [A-] = 0.025


    [HA] = 0.0025



    Step 2 :  

    Substitute the values in the formula.


    pH = -log[Ka] + log$\frac{[A^-]}{[HA]}$


    pH = -log[4.2 $\times$ 10-7] + log$\frac{0.025}{0.0025}$


    pH = 7.207



    Answer  :  

    The answer is pH = 7.207



  2. Calculate the pH of blood containing 0.0025M HPO42- and 0.0015M H2PO4-. Ka of H2PO4- = 6.2 $\times$ 10-8.


    Step 1 :  

    Given data


    Ka = 6.2 $\times$ 10-8


    [A-] = 0.0025


    [HA] = 0.0015



    Step 2 :  

    Substitute the values in the formula.


    pH = -log[Ka] + log$\frac{[A^-]}{[HA]}$


    pH = -log[6.2 $\times$ 10-8] + log$\frac{[0.0025]}{[0.0015]}$


    pH = 7.42



    Answer  :  

    The answer is pH = 7.42



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